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- Title
髋骨性关节炎滑膜组织中钙结合蛋白 (S100A12) 的表达及意义.
- Authors
代凤雷; 孙强; 张斌; 金雯; 陈荣国; 钱建吉
- Abstract
Objective To investigate the expression of calcium binding protein S100A12 in synovial tissue and serum of hip osteoarthritis, to study its role in the hip osteoarthritis pathogenesis, and to provide theoretical and experimental evidence. Methods Twenty-seven hip osteoarthritis patients for hip replacement (OA group) and 35 cases of femoral neck fracture patients (control group) were studied. The progression of osteoarthritis was classified with Kellgren- Lawrence grading scale by evaluating X-ray images of the hip joint. Serum samples and synovial tissue from two groups were collected before and during operation. S100A12 content and expression were analyzed using enzyme-linked immunosorbent assay (ELISA) and immunohistochemical (IHC) staining. Results Serum S100A12 levels in OA group (94.20±49.85) ng/ml were higher than that in control group (25.32±16.87) ng/mL (P<0.01). The S100A12 content in synovial tissue of OA group (3.73±1.97 ng/mg total protein) was higher than that in controls (1.67.±1.21 ng/mg total protein, P<0.01). IHC analysis showed that S100A12 was positive in the cytoplasm of neutrophils in the synovial capillaries, as well as in the cytoplasm of some synovial cells. The mean optic density (MOD) in OA group (0.21±0.06) was significantly higher than that in control group (0.13±0.05, P<0.01). The level of S100A12 in serum of grade 4 by K-L grading scales (104.81 + 46.68 ng/ml) was significantly higher than that in the level of grade 3 (86.85 + 52.46 ng/mL, P<0.01). The level of S100A12 in synovial tissue of grade 4 (4.62 + 1.95 ng/mg total protein) was significantly higher than that in the level of grade 3 (3.13 + 1.82 ng/mg total protein, P<0.01). Conclusion The expression of S100A12 significantly increases in synovial tissue of patients with hip osteoarthritis. The levels of S100A12 are closely related to the severity of hip osteoarthritis, which can be used as an effective biochemical marker for early detection of osteoarthritis and the evaluation of clinical therapeutic of OA.
- Subjects
OSTEOARTHRITIS; CALCIUM-binding proteins
- Publication
Chinese Journal of Osteoporosis, 2017, Vol 23, Issue 1, p27
- ISSN
1006-7108
- Publication type
Article
- DOI
10.3969/j.issn.1006-7108.2017.01.006