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- Title
A simple, noninvasive and efficient method for transdermal delivery of siRNA.
- Authors
Lin, Chang-Min; Huang, Keng; Zeng, Yang; Chen, Xian-Cai; Wang, Sen; Li, Yu
- Abstract
Effective delivery of therapeutic agents is the most challenging hurdle in the use of RNA interference for research and in the clinic. Here, we assessed whether a short synthetic peptide, ACSSSPSKHCG (TD-1), could be transported through rat footpad (follicle-free) skin and efficiently deliver small interfering RNA (siRNA) to knock down a target gene. Fluorescence microscopy revealed that topical co-administration of FITC-labeled TD-1 and FAM-labeled siRNA distributed uniformly from the epidermis to the subcutaneous tissue of rat footpad skin. Transmission electron microscopy revealed the absence of cell-cell junctions and enlarged spaces between epithelial cells in the TD-1-treated footpad skin. TD-1 delivery of anti-GAPDH siRNA significantly reduced the level of GAPDH in 72 h. TD-1 can create a transient opening in non-follicle rat skin for delivery of siRNA and reveal a novel mechanism of transdermal delivery of TD-1 and siRNA into the epidermis for gene knockdown. The system might have potential for siRNA delivery in skin for drug therapy.
- Subjects
SMALL interfering RNA; DRUG delivery systems; PEPTIDES; FLUORESCENCE microscopy; CELL junctions; EPITHELIAL cells; EPIDERMIS
- Publication
Archives of Dermatological Research, 2012, Vol 304, Issue 2, p139
- ISSN
0340-3696
- Publication type
Article
- DOI
10.1007/s00403-011-1181-5