We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Detection Of Circulating Neonatal Nav1.5 (Nnav1.5) Antigen In The Blood Of 4T1 Orthotopic Breast Cancer Mice Model And Breast Cancer Patients.
- Authors
Rajaratinam, Harishini; Rasudin, Nur Syahmina; Safuan, Sabreena; Abdullah, Nurul Asma; Mokhtar, Noor Fatmawati; Yahya, Maya Mazuwin; Wan Zain, Wan Zainira; Damitri Al-Astani, Tengku Ahmad; Mohd Fuad, Wan Ezumi
- Abstract
Introduction: Neonatal Nav1.5 (nNav1.5) is the alternative splice variant of Nav1.5 and its role in progression of metastasis is well established. Initially, the detection of nNav1.5 was performed on breast cancer tissues. However, this approach is highly invasive and expensive, therefore, we have attempted to detect the presence of circulating nNav1.5 in whole blood. Methods: A total of 16 female BALB/c mice was divided equally into two groups: control group (n=8) and 4T1 orthotopic mice models (n=8). 4T1 cells were injected at the 3rd mammary fat pad of the 4T1 mice group whereas PBS was introduced at the same site for the control group. After 42 days of tumour development, all mice were sacrificed. Blood samples and organs were collected. Organs were subjected to histopathological analysis. Simultaneously, 64 healthy participants and 64 breast cancer patients were recruited. Blood was withdrawn and collected. RNA extraction was performed on both animal and human blood using respective kits. RT-qPCR was performed using nNav1.5 and β-actin housekeeping genes on both mice and human samples. The relative mRNA expression of target genes was calculated using the 2−ΔCt quantitative method for each individual data point. Results: Histopathological analysis revealed metastasis to the heart, lungs, liver, spleen, kidneys, and ribcages of 4T1 mice. nNav1.5 antigen was only detected in the whole blood of 4T1 mice and not in control mice. The 2−ΔCt values ranged from 0.0001-0.0106. However, only five blood samples from the group of breast cancer patients exhibited the presence of circulating nNav1.5 antigen whereby the 2−ΔCt values ranged from 0.0121-1.1728. Additionally, there was no detectable presence of nNav1.5 in the samples of healthy participants. Conclusion: The presence of circulating nNav1.5 could be detected in the whole blood of 4T1 orthotopic mice, but the expression could be too low in the blood of breast cancer patients.
- Subjects
BREAST cancer; CANCER patients; ANIMAL disease models; LABORATORY mice; ANTIGENS
- Publication
Malaysian Journal of Medicine & Health Sciences, 2022, Vol 18, p54
- ISSN
1675-8544
- Publication type
Abstract