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- Title
Nonredundant functions of <italic>Arabidopsis</italic> LecRK‐V.2 and LecRK‐VII.1 in controlling stomatal immunity and jasmonate‐mediated stomatal closure.
- Authors
Yekondi, Shweta; Liang, Fu‐Chun; Okuma, Eiji; Radziejwoski, Amandine; Mai, Hsien‐Wei; Swain, Swadhin; Singh, Prashant; Gauthier, Mathieu; Chien, Hsiao‐Chiao; Murata, Yoshiyuki; Zimmerli, Laurent
- Abstract
Summary: Stomatal immunity restricts bacterial entry to leaves through the recognition of microbe‐associated molecular patterns (MAMPs) by pattern‐recognition receptors (PRRs) and downstream abscisic acid and salicylic acid signaling. Through a reverse genetics approach, we characterized the function of the L‐type lectin receptor kinase‐V.2 (LecRK‐V.2) and ‐VII.1 (LecRK‐VII.1). Analyses of interactions with the PRR FLAGELLIN SENSING2 (FLS2) were performed by co‐immunoprecipitation and bimolecular fluorescence complementation and whole‐cell patch‐clamp analyses were used to evaluate guard cell Ca2+‐permeable cation channels. The <italic>Arabidopsis thaliana</italic> LecRK‐V.2 and LecRK‐VII.1 and notably their kinase activities were required for full activation of stomatal immunity. Knockout <italic>lecrk‐V.2</italic> and <italic>lecrk‐VII.1</italic> mutants were hyper‐susceptible to <italic>Pseudomonas syringae</italic> infection and showed defective stomatal closure in response to bacteria or to the MAMPs flagellin and EF‐Tu. By contrast, <italic>Arabidopsis</italic> over‐expressing <italic>LecRK‐V.2</italic> or <italic>LecRK‐VII.1</italic> demonstrated a potentiated stomatal immunity. LecRK‐V.2 and LecRK‐VII.1 are shown to be part of the FLS2 PRR complex. In addition, LecRK‐V.2 and LecRK‐VII.1 were critical for methyl jasmonate (MeJA)‐mediated stomatal closure, notably for MeJA‐induced activation of guard cell Ca2+‐permeable cation channels. This study highlights the role of LecRK‐V.2 and LecRK‐VII.1 in stomatal immunity at the FLS2 PRR complex and in MeJA‐mediated stomatal closure.
- Subjects
MICROORGANISMS; PATTERN perception receptors; ABSCISIC acid; SALICYLIC acid; PLANT genetics; ARABIDOPSIS
- Publication
New Phytologist, 2018, Vol 218, Issue 1, p253
- ISSN
0028-646X
- Publication type
Article
- DOI
10.1111/nph.14953