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- Title
Immulectin-4 from the tobacco hornworm Manduca sexta binds to lipopolysaccharide and lipoteichoic acid.
- Authors
Yu, X.-Q.; Ling, E.; Tracy, M. E.; Zhu, Y.
- Abstract
Insect C-type lectins function as pattern recognition receptors in innate immunity. In the tobacco hornworm Manduca sexta, we have previously isolated three C-type lectins named immulectins, which are involved in innate immune responses. Here, we report a new member of the immulectin family, immulectin-4 (IML-4). IML-4 mRNA was detected in the fat body of control larvae and was induced in the fat body when larvae were injected with bacteria. Recombinant IML-4 bound to bacterial lipopolysaccharide (LPS) and lipoteichoic acid (LTA), and the binding activity was not affected by addition of calcium or EGTA. IML-4 agglutinated bacteria and yeast, and agglutination of Escherichia coli by IML-4 was concentration- and calcium-dependent. IML-4 also enhanced haemocyte encapsulation and melanization.
- Subjects
TOBACCO hornworm; POLYSACCHARIDES; LECTINS; IMMUNE response; ENDOTOXINS; BLOOD cells
- Publication
Insect Molecular Biology, 2006, Vol 15, Issue 2, p119
- ISSN
0962-1075
- Publication type
Article
- DOI
10.1111/j.1365-2583.2006.00618.x