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- Title
Anti-Hepatitis B Virus Effects of Dehydrocheilanthifoline from Corydalis saxicola.
- Authors
Zeng, Fan-Li; Xiang, Yang-Fei; Liang, Zhen-Ran; Wang, Xiao; Huang, Dan-e; Zhu, Sui-Nan; Li, Min-Min; Yang, De-Po; Wang, Dong-Mei; Wang, Yi-Fei
- Abstract
In this report, the anti-hepatitis B virus (HBV) activity of dehydrocheilanthifoline (DHCH), a quaternary ammonium alkaloid isolated from the traditional Chinese medicine Corydalis saxicola Bunting (Papaveraceae), was determined in vitro. Following six days of treatment, DHCH efficiently suppressed the secretions of HBsAg and HBeAg in HepG2.2.15 cell cultures, with a half-maximal inhibitory concentration (IC50) of 15.84 and 17.12 μM, and with a therapeutic index (TI) of 7.32 and 6.77, respectively. Further studies revealed that DHCH reduced the levels of extracellular DNA, intracellular DNA and covalently closed circular DNA (cccDNA) of HBV in a dose-dependent and time-dependent manner, with IC50 values of 15.08, 7.62 and 8.25 μM, respectively after six days of treatment. In contrast, the level of viral pre-genomic RNA (pgRNA) increased 6.13-fold after treatment with DHCH. Together, it was demonstrated for the first time that DHCH could significantly inhibit the replication of HBV, which warrants further studies on the antiviral mechanisms of DHCH, and suggests that it may be a promising candidate in the therapy of HBV infection.
- Subjects
DNA analysis; RNA analysis; ALKALOIDS; ANALYSIS of variance; BIOLOGICAL assay; CELL culture; ENZYME-linked immunosorbent assay; HEPATITIS B; CHINESE medicine; MOLECULAR structure; POLYMERASE chain reaction; RESEARCH funding; T-test (Statistics); TOXICITY testing; REPEATED measures design; DATA analysis software; DESCRIPTIVE statistics; IN vitro studies
- Publication
American Journal of Chinese Medicine, 2013, Vol 41, Issue 1, p119
- ISSN
0192-415X
- Publication type
Article
- DOI
10.1142/S0192415X13500092