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- Title
Next-generation rapid serum tube technology using prothrombin activator coagulant: fast, high-quality serum from normal samples.
- Authors
Zhao, Kong-Nan; Dimeski, Goce; de Jersey, John; Johnson, Lambro A.; Grant, Michael; Masci, Paul P.; Lavin, Martin F.
- Abstract
Background: Incomplete blood clotting or latent clotting in serum is a common laboratory problem, especially for patients on anticoagulant therapy or when serum tubes are centrifuged before clotting is completed. We describe a novel approach to producing high-quality serum using snake venom prothrombin activator complex (OsPA) as an additive in blood collection tubes for non-anticoagulated (normal) individuals. Methods: Plasma clotting assays were performed using a Hyland-Clotek instrument. Blood clotting was visually observed, and thromboelastography was also performed to determine the important parameters of coagulation. Thrombin generation was assayed using the chromogenic substrate S-2238, and biochemical analytes in the serum were determined on chemistry and immunoassay analysers. Fibrinogen was determined by either ELISA or Clauss fibrinogen assay. Results: We initially showed that OsPA had strong coagulation activity in clotting not only recalcified citrated plasma and recalcified citrated whole blood, but also fresh whole blood in a clinical setting. The use of TEG clearly showed improved speed of clotting and generation of a firmer clot. We also showed that the use of OsPA to produce serum did not interfere with the determination of commonly measured biochemical analytes. The underlying clotting mechanism involves a burst of thrombin production at the initial stages of the clotting process upon contact with prothrombin in blood. Conclusions: These results demonstrate rapid generation of high-quality serum, contributing to faster turnaround times with standardised quality samples, for accurate analyte determinations in normal individuals.
- Subjects
BLOOD coagulation; SERUM; PROTHROMBIN; BLOOD coagulants; BLOOD plasma
- Publication
Clinical Chemistry & Laboratory Medicine, 2019, Vol 57, Issue 4, p483
- ISSN
1434-6621
- Publication type
Article
- DOI
10.1515/cclm-2018-0397