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- Title
Highly Sensitive Electrochemical Sensor for Diagnosis of Diabetic Ketoacidosis (DKA) by Measuring Ketone Bodies in Urine.
- Authors
Go, Anna; Park, Sung Ryul; Ku, Yejin; Sun, Mingge; Yeon, Sangho; Lee, Jin-Kyun; Lee, Sang Wook; Lee, Min-Ho
- Abstract
In this report, we present an enzyme deposited Au electrode for an electrochemical measurement of acetylacetic acid (AcAc) in urine. The electrode has an immobilized layer of a mixture of D-β-hydroxybutyrate dehydrogenase (HBDH) and nicotinamide adenine dinucleotide (NADH) as sensing material to investigate its electroanalytical properties by means of cyclic voltammetry (CV). The modified electrodes are used for the detection of AcAc and present a linear current increase when the AcAc concentration increases. The electrode presents a limit of detection (LOD) of 6.25 mg/dL in the range of 6.25–100 mg/dL for investigation of clinical relevance. Finally, the electrode was evaluated using 20 patient samples. The measured results of urine ketone by the developed electrode were compared with the clinical results from a commercial kit, and the analysis showed good agreement. The proposed electrode was demonstrated to be a very promising platform as a miniaturized electrochemical analyzer for point-of-care monitoring of the critical biochemical parameters such as urine ketone.
- Subjects
ELECTROCHEMICAL sensors; DIABETIC acidosis; ELECTROCHEMICAL electrodes; KETONES; URINE; BIOELECTROCHEMISTRY
- Publication
Sensors (14248220), 2021, Vol 21, Issue 14, p4902
- ISSN
1424-8220
- Publication type
Article
- DOI
10.3390/s21144902