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- Title
Highly Sensitive and Practical Detection of Plant Viruses via Electrical Impedance of Droplets on Textured Silicon-Based Devices.
- Authors
Ambrico, Marianna; Ambrico, Paolo Francesco; Minafra, Angelantonio; De Stradis, Angelo; Vona, Danilo; Cicco, Stefania R.; Palumbo, Fabio; Favia, Pietro; Ligonzo, Teresa
- Abstract
Early diagnosis of plant virus infections before the disease symptoms appearance may represent a significant benefit in limiting disease spread by a prompt application of appropriate containment steps. We propose a label-free procedure applied on a device structure where the electrical signal transduction is evaluated via impedance spectroscopy techniques. The device consists of a droplet suspension embedding two representative purified plant viruses i.e., Tomato mosaic virus and Turnip yellow mosaic virus, put in contact with a highly hydrophobic plasma textured silicon surface. Results show a high sensitivity of the system towards the virus particles with an interestingly low detection limit, from tens to hundreds of attomolar corresponding to pg/mL of sap, which refers, in the infection time-scale, to a concentration of virus particles in still-symptomless plants. Such a threshold limit, together with an envisaged engineering of an easily manageable device, compared to more sophisticated apparatuses, may contribute in simplifying the in-field plant virus diagnostics.
- Subjects
PLANT viruses; ELECTRIC impedance; IMPEDANCE spectroscopy; HYDROPHOBIC compounds; MOSAIC viruses
- Publication
Sensors (14248220), 2016, Vol 16, Issue 11, p1946
- ISSN
1424-8220
- Publication type
Article
- DOI
10.3390/s16111946