We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Improved survival in simulated surgical infection with combined cytokine, antibiotic and immunostimulant therapy.
- Authors
Gaar, E.; Naziri, W.; Cheadle, W. G.; Pietsch, J. D.; Johnson, M.; Polk, H. C.
- Abstract
A study was performed to find an ideal combination and sequence of cytokines, antibiotics and immunorestorative agents to enhance survival from serious infection. The effects of combinations of granulocyte-macrophage colony-stimulating factor (GM-CSF), tumour necrosis factor (TNF) a, the immune adjuvant muramyl dipeptide (MDP) and two systemic antibiotics were studied in a validated murine model of surgical infection. A single cotton suture containing absorbed Klebsiella pneumoniae was placed into the thighs of mice to produce local and systemic infection. Control mice received a volume of subcutaneous saline equal to that of the therapeutic agent; only 18 per cent survived 9 days after infection. The survival time of mice treated with any single agent was similar to that of controls. The group given maximal combined therapy (65 mice) received GM-CSF, TNF-a, MDP, and ampicillinsulbactam or cefoxitin for 6 days. The survival rate in this group 9 days after the introduction of infection was 84-90 per cent (P< 0·0001), suggesting that specific combinations of cytokines, immunostimulants and antibiotics may be useful in combating lethal infection.
- Publication
British Journal of Surgery, 1994, Vol 81, Issue 9, p1309
- ISSN
0007-1323
- Publication type
Article
- DOI
10.1002/bjs.1800810915