We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
The Phaseolus vulgaris Receptor-Like Kinase PvFER1 and the Small Peptides PvRALF1 and PvRALF6 Regulate Nodule Number as a Function of Nitrate Availability.
- Authors
Solís-Miranda, Jorge; Juárez-Verdayes, Marco A.; Nava, Noreide; Rosas, Paul; Leija-Salas, Alfonso; Cárdenas, Luis; Quinto, Carmen
- Abstract
Legumes associate with Gram-negative soil bacteria called rhizobia, resulting in the formation of a nitrogen-fixing organ, the nodule. Nodules are an important sink for photosynthates for legumes, so these plants have developed a systemic regulation mechanism that controls their optimal number of nodules, the so-called autoregulation of nodulation (AON) pathway, to balance energy costs with the benefits of nitrogen fixation. In addition, soil nitrate inhibits nodulation in a dose-dependent manner, through systemic and local mechanisms. The CLE family of peptides and their receptors are key to tightly controlling these inhibitory responses. In the present study, a functional analysis revealed that PvFER1, PvRALF1, and PvRALF6 act as positive regulators of the nodule number in growth medium containing 0 mM of nitrate but as negative regulators in medium with 2 and 5 mM of nitrate. Furthermore, the effect on nodule number was found to be consistent with changes in the expression levels of genes associated with the AON pathway and with the nitrate-mediated regulation of nodulation (NRN). Collectively, these data suggest that PvFER1, PvRALF1, and PvRALF6 regulate the optimal number of nodules as a function of nitrate availability.
- Subjects
COMMON bean; LEGUMES; NITROGEN fixation; PEPTIDES; NITRATES; SOIL microbiology
- Publication
International Journal of Molecular Sciences, 2023, Vol 24, Issue 6, p5230
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms24065230