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- Title
An Innovative Customized Biomimetic Hydrogel for Drug Screening Application Potential: Biocompatibility and Cell Invasion Ability.
- Authors
Ou, Keng-Liang; Huang, Chiung-Fang; Lan, Wen-Chien; Huang, Bai-Hung; Pan, Hsu-An; Shen, Yung-Kang; Saito, Takashi; Tsai, Hsin-Yu; Cho, Yung-Chieh; Hung, Kuo-Sheng; Chou, Hsin-Hua
- Abstract
The ability of Pluronic F127 (PF127) conjugated with tetrapeptide Gly-Arg-Gly-Asp (GRGD) as a sequence of Arg-Gly-Asp (RGD) peptide to form the investigated potential hydrogel (hereafter referred to as 3DG bioformer (3BE)) to produce spheroid, biocompatibility, and cell invasion ability, was assessed in this study. The fibroblast cell line (NIH 3T3), osteoblast cell line (MG-63), and human breast cancer cell line (MCF-7) were cultured in the 3BE hydrogel and commercial product (Matrigel) for comparison. The morphology of spheroid formation was evaluated via optical microscopy. The cell viability was observed through cell counting Kit-8 assay, and cell invasion was investigated via Boyden chamber assay. Analytical results indicated that 3BE exhibited lower spheroid formation than Matrigel. However, the 3BE appeared biocompatible to NIH 3T3, MG-63, and MCF-7 cells. Moreover, cell invasion ability and cell survival rate after invasion through the 3BE was displayed to be comparable to Matrigel. Thus, these findings demonstrate that the 3BE hydrogel has a great potential as an alternative to a three-dimensional cell culture for drug screening applications.
- Subjects
NATIONAL Institutes of Health (U.S.); CYTOCOMPATIBILITY; HYDROGELS; PEPTIDES; CELL culture; CELL lines; BIOMIMETIC materials
- Publication
International Journal of Molecular Sciences, 2022, Vol 23, Issue 3, p1488
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms23031488