We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Pan-Genome Reverse Vaccinology Approach for the Design of Multi-Epitope Vaccine Construct against Escherichia albertii.
- Authors
Jalal, Khurshid; Khan, Kanwal; Ahmad, Diyar; Hayat, Ajmal; Basharat, Zarrin; Abbas, Muhammad Naseer; Alghamdi, Saad; Almehmadi, Mazen; Sahibzada, Muhammad Umar Khayam
- Abstract
Escherichia albertii is characterized as an emerging pathogen, causing enteric infections. It is responsible for high mortality rate, especially in children, elderly, and immunocompromised people. To the best of our knowledge, no vaccine exists to curb this pathogen. Therefore, in current study, we aimed to identify potential vaccine candidates and design chimeric vaccine models against Escherichia albertii from the analysis of publicly available data of 95 strains, using a reverse vaccinology approach. Outer-membrane proteins (n = 4) were identified from core genome as vaccine candidates. Eventually, outer membrane Fimbrial usher (FimD) protein was selected as a promiscuous vaccine candidate and utilized to construct a potential vaccine model. It resulted in three epitopes, leading to the design of twelve vaccine constructs. Amongst these, V6 construct was found to be highly immunogenic, non-toxic, non-allergenic, antigenic, and most stable. This was utilized for molecular docking and simulation studies against six HLA and two TLR complexes. This construct can therefore be used for pan-therapy against different strains of E. albertii and needs to be tested in vitro and in vivo.
- Subjects
ESCHERICHIA; INTESTINAL infections; MOLECULAR docking; VACCINES; DEATH rate
- Publication
International Journal of Molecular Sciences, 2021, Vol 22, Issue 23, p12814
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms222312814