We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Calycosin-7-O-β-Glucoside Isolated from Astragalus membranaceus Promotes Osteogenesis and Mineralization in Human Mesenchymal Stem Cells.
- Authors
Park, Kyung-Ran; Park, Ji Eun; Kim, Bomi; Kwon, Il Keun; Hong, Jin Tae; Yun, Hyung-Mun
- Abstract
Stem cells have received attention in various diseases, such as inflammatory, cancer, and bone diseases. Mesenchymal stem cells (MSCs) are multipotent stem cells that are critical for forming and repairing bone tissues. Herein, we isolated calycosin-7-O-β-glucoside (Caly) from the roots of Astragalus membranaceus, which is one of the most famous medicinal herbs, and investigated the osteogenic activities of Caly in MSCs. Caly did not affect cytotoxicity against MSCs, whereas Caly enhanced cell migration during the osteogenesis of MSCs. Caly increased the expression and enzymatic activities of ALP and the formation of mineralized nodules during the osteogenesis of MSCs. The osteogenesis and bone-forming activities of Caly are mediated by bone morphogenetic protein 2 (BMP2), phospho-Smad1/5/8, Wnt3a, phospho-GSK3β, and phospho-AKT, inducing the expression of runt-related transcription factor 2 (RUNX2). In addition, Caly-mediated osteogenesis and RUNX2 expression were attenuated by noggin and wortmannin. Moreover, the effects were validated in pre-osteoblasts committed to the osteoblast lineages from MSCs. Overall, our results provide novel evidence that Caly stimulates osteoblast lineage commitment of MSCs by triggering RUNX2 expression, suggesting Caly as a potential anabolic drug to prevent bone diseases.
- Subjects
HUMAN stem cells; MESENCHYMAL stem cells; ASTRAGALUS membranaceus; MULTIPOTENT stem cells; BONE morphogenetic proteins
- Publication
International Journal of Molecular Sciences, 2021, Vol 22, Issue 21, p11362
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms222111362