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- Title
Applications of Engineered DNA-Binding Molecules Such as TAL Proteins and the CRISPR/Cas System in Biology Research.
- Authors
Toshitsugu Fujita; Hodaka Fujii
- Abstract
Engineered DNA-binding molecules such as transcription activator-like effector (TAL or TALE) proteins and the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas) (CRISPR/Cas) system have been used extensively for genome editing in cells of various types and species. The sequence-specific DNA-binding activities of these engineered DNA-binding molecules can also be utilized for other purposes, such as transcriptional activation, transcriptional repression, chromatin modification, visualization of genomic regions, and isolation of chromatin in a locus-specific manner. In this review, we describe applications of these engineered DNA-binding molecules for biological purposes other than genome editing.
- Subjects
DNA-binding proteins; CRISPRS; MOLECULAR structure of chromatin; GENOME editing; ZINC-finger proteins; NUCLEOTIDE sequencing
- Publication
International Journal of Molecular Sciences, 2015, Vol 16, Issue 10, p23143
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms161023143