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- Title
Potential use of new diphenylurea derivatives in micropropagation of Capparis spinosa L.
- Authors
Carra, Angela; Del Signore, Maria; Sottile, Francesco; Ricci, Ada; Carimi, Francesco
- Abstract
A protocol for in vitro multiplication of caper ( Capparis spinosa L. subsp. rupestris) from nodal segments collected from mature plants was developed. For shoot multiplication, one auxin (indol-3-butyric acid, IBA) and cytokinins of two different classes were used: the N6-substituted adenine derivatives 6-benzylamino purine (BAP), and the two synthetic phenylurea derivatives N-phenyl- N′-benzothiazol-6-ylurea (PBU) and N-phenyl- N′-(1,2,3-thidiazol-5-yl) urea (thidiazuron, TDZ). Maximum shoot production was achieved from explants cultured with the adeninic cytokinin BAP (4 μM) and the auxin IBA (0.5 μM). New shoots longer than 1 cm were used for rooting. To induce root formation, three auxins [indole-3-butyric acid (IBA), 1-naphthaleneacetic acid (NAA) and 3-Indoleacetic acid (IAA)] and two synthetic phenylurea derivatives [ N, N-bis-(2,3-methylenedioxyphenyl)urea (2,3-MDPU) and N, N-bis-(3,4-methylenedioxyphenyl)urea (3,4-MDPU)] were used. All rooting compounds tested stimulated the formation of roots. However, the best result in terms of a high percentage of rooted shoots having a well-developed root system with many lateral roots was achieved with the synthetic phenylurea 2,3-MDPU (1 μM) with 93.7% of well rooted plantlets. About 80% of rooted plantlets were successfully acclimatized and transferred to the greenhouse.
- Subjects
CAPPARIS spinosa; BUTYRIC acid; AUXIN; PLANT hormones; PHENYLUREA compounds; PLANT growth promoting substances
- Publication
Plant Growth Regulation, 2012, Vol 66, Issue 3, p229
- ISSN
0167-6903
- Publication type
Article
- DOI
10.1007/s10725-011-9645-3