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- Title
In vitro and in vivo evaluation of Bola-surfactant containing niosomes for transdermal delivery.
- Authors
Donatella Paolino; Rita Muzzalupo; Antonio Ricciardi; Christian Celia; Nevio Picci; Massimo Fresta
- Abstract
Abstract  A novel niosome formulation is proposed for topical drug delivery of ammonium glycyrrhizinate, a natural compound with an efficacious anti-inflammatory activity. Niosomes were made up of a new non ionic surfactant, α,Ï-hexadecyl-bis-(1-aza-18-crown-6) (Bola-surfactant)-Span 80-cholesterol (2:3:1 molar ratio). Niosome vesicles were prepared with the thin layer evaporation method and were physico-chemically characterized. The tolerability of Bola-surfactant both as free molecules or assembled ion niosome vesicles was evaluated in vitro on cultured of human keratinocyte cells (NCTC2544). Human tolerability was evaluated on volunteers. The ability of Bola-niosomes to promote intracellular delivery was evaluated by confocal laser scanning microscopy (CLSM) studies. Human stratum corneum and epidermis (SCE) membranes were used in vitro to investigate the percutaneous permeation. The anti-inflammatory activity of ammonium glycyrrhizinate was evaluated in vivo on human volunteers with a chemically induced erythema. Experimental data show that Bola-niosomes are characterized by a mean size of â¼400 nm and are able to provide an encapsulation efficiency of 40% with respect to the drug amount used during preparation. CLSM showed that Bola-niosomes were able to promote the intracellular uptake of the delivered substances. Bola-niosomes were also able to significantly improve (p in vitro and in vivo. Ammonium glycyrrhizinate-loaded Bola-niosomes determined a significant (p in vivo anti-inflammatory activity of the drug. An effective example of conjugating innovative colloidal carriers, coming from pharmaceutical nanotechnology, and therapeutically effective natural compounds, coming from traditional medicine, was reported.
- Subjects
TRANSDERMAL medication; SURFACE active agents; CONFOCAL microscopy; NANOTECHNOLOGY
- Publication
Biomedical Microdevices, 2007, Vol 9, Issue 4, p421
- ISSN
1387-2176
- Publication type
Article
- DOI
10.1007/s10544-007-9046-6