We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Temporal and spatial cellular and molecular pathological alterations with single-cell resolution in the adult spinal cord after injury.
- Authors
Li, Chen; Wu, Zhourui; Zhou, Liqiang; Shao, Jingliang; Hu, Xiao; Xu, Wei; Ren, Yilong; Zhu, Xingfei; Ge, Weihong; Zhang, Kunshan; Liu, Jiping; Huang, Runzhi; Yu, Jing; Luo, Dandan; Yang, Xuejiao; Zhu, Wenmin; Zhu, Rongrong; Zheng, Changhong; Sun, Yi Eve; Cheng, Liming
- Abstract
Spinal cord injury (SCI) involves diverse injury responses in different cell types in a temporally and spatially specific manner. Here, using single-cell transcriptomic analyses combined with classic anatomical, behavioral, electrophysiological analyses, we report, with single-cell resolution, temporal molecular and cellular changes in crush-injured adult mouse spinal cord. Data revealed pathological changes of 12 different major cell types, three of which infiltrated into the spinal cord at distinct times post-injury. We discovered novel microglia and astrocyte subtypes in the uninjured spinal cord, and their dynamic conversions into additional stage-specific subtypes/states. Most dynamic changes occur at 3-days post-injury and by day-14 the second wave of microglial activation emerged, accompanied with changes in various cell types including neurons, indicative of the second round of attacks. By day-38, major cell types are still substantially deviated from uninjured states, demonstrating prolonged alterations. This study provides a comprehensive mapping of cellular/molecular pathological changes along the temporal axis after SCI, which may facilitate the development of novel therapeutic strategies, including those targeting microglia.
- Publication
Signal Transduction & Targeted Therapy, 2022, Vol 6, Issue 1, p1
- ISSN
2095-9907
- Publication type
Article
- DOI
10.1038/s41392-022-00885-4