We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Whole-Genome Sequencing and Comparative Genome Analysis Provided Insight into the Predatory Features and Genetic Diversity of Two Bdellovibrio Species Isolated from Soil.
- Authors
Oyedara, Omotayo Opemipo; Segura-Cabrera, Aldo; Guo, Xianwu; Elufisan, Temidayo Oluyomi; Cantú González, Rafael Alejandro; Rodríguez Pérez, Mario A.
- Abstract
<italic>Bdellovibrio</italic> spp. are predatory bacteria with great potential as antimicrobial agents. Studies have shown that members of the genus <italic>Bdellovibrio</italic> exhibit peculiar characteristics that influence their ecological adaptations. In this study, whole genomes of two different <italic>Bdellovibrio</italic> spp. designated SKB1291214 and SSB218315 isolated from soil were sequenced. The core genes shared by all the <italic>Bdellovibrio</italic> spp. considered for the pangenome analysis including the epibiotic <italic>B. exovorus</italic> were 795. The number of unique genes identified in <italic>Bdellovibrio</italic> spp. SKB1291214, SSB218315, W, and <italic>B. exovorus</italic> JJS was 1343, 113, 857, and 1572, respectively. These unique genes encode hydrolytic, chemotaxis, and transporter proteins which might be useful for predation in the <italic>Bdellovibrio</italic> strains. Furthermore, the two <italic>Bdellovibrio</italic> strains exhibited differences based on the % GC content, amino acid identity, and 16S rRNA gene sequence. The 16S rRNA gene sequence of <italic>Bdellovibrio</italic> sp. SKB1291214 shared 99% identity with that of an uncultured <italic>Bdellovibrio</italic> sp. clone 12L 106 (a pairwise distance of 0.008) and 95–97% identity (a pairwise distance of 0.043) with that of other culturable terrestrial <italic>Bdellovibrio</italic> spp., including strain SSB218315. In <italic>Bdellovibrio</italic> sp. SKB1291214, 174 bp sequence was inserted at the host interaction <italic>(hit)</italic> locus region usually attributed to prey attachment, invasion, and development of host independent <italic>Bdellovibrio</italic> phenotypes. Also, a gene equivalent to <italic>Bd0108</italic> in <italic>B. bacteriovorus</italic> HD100 was not conserved in <italic>Bdellovibrio</italic> sp. SKB1291214. The results of this study provided information on the genetic characteristics and diversity of the genus <italic>Bdellovibrio</italic> that can contribute to their successful applications as a biocontrol agent.
- Subjects
NUCLEOTIDE sequencing; BDELLOVIBRIO; BACTERIA; ANTI-infective agents; GENOMICS
- Publication
International Journal of Genomics, 2018, p1
- ISSN
2314-436X
- Publication type
Article
- DOI
10.1155/2018/9402073