We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Rapid, Affordable and Efficient Screening of Multiple Blood Abnormalities Made Possible Using an Automated Tool for MALDI-ToF Spectrometry Analysis.
- Authors
Pais, Ricardo J.; Jardine, Christian; Zmuidinaite, Raminta; Lacey, Jonathan; Butler, Steve; Iles, Ray
- Abstract
Featured Application: Population screening for blood abnormalities suggestive of haemoglobinopathies, thalassemias and pre-diabetes/diabetes in national health care control programs of Asian, Middle East and African countries. Screening programs for genetic and metabolic diseases such as haemoglobinopathies, thalassemias and diabetes are a worldwide problem that faces economic and technological limitations. This is mainly because genetic and metabolic tests are too expensive and time consuming to be implemented. MALDI-ToF mass spectrometry is a rapid and affordable high throughput technique with diagnostic potential for these diseases but still constrained by the timing and complexity of data analysis. To overcome this technological limitation, we developed a fully automated software solution in our MALDI-ToF instrument towards the detection of haemoglobinopathies, thalassemias and diabetes on one blood card sample. The software was tested for its efficiency and accuracy on 171 blood samples rendering 30-fold faster analysis with less bias and rounding errors in comparison with the manual approach. In this study, we identified the variability associated with the disease biomarkers in healthy individuals and successfully applied predictive models to detect blood abnormalities. Taken together, we demonstrated in this study that population screening of multiple blood disorders is made possible using MALDI-ToF technology in combination with automated software tools.
- Subjects
MIDDLE East; NATIONAL health services; GENETIC testing; SPECTROMETRY; BLOOD; SOFTWARE development tools
- Publication
Applied Sciences (2076-3417), 2019, Vol 9, Issue 23, p4999
- ISSN
2076-3417
- Publication type
Article
- DOI
10.3390/app9234999