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- Title
Suppression of Midgut Microbiota Impact Pyrethroid Susceptibility in Aedes aegypti.
- Authors
Gómez-Govea, Mayra A.; de Lourdes Ramírez-Ahuja, María; Contreras-Perera, Yamili; Jiménez-Camacho, Armando J.; Ruiz-Ayma, Gabriel; Villanueva-Segura, Olga Karina; de Jesús Trujillo-Rodríguez, Gerardo; Delgado-Enciso, Iván; Martínez-Fierro, Margarita L.; Manrique-Saide, Pablo; Puerta-Guardo, Henry; Flores-Suárez, Adriana E.; Ponce-García, Gustavo; Rodríguez-Sánchez, Iram P.
- Abstract
Aedes aegypti is a mosquito that transmits viral diseases such as dengue, chikungunya, Zika, and yellow fever. The insect's microbiota is recognized for regulating several biological processes, including digestion, metabolism, egg production, development, and immune response. However, the role of the bacteria involved in insecticide susceptibility has not been established. Therefore, the objective of this study was to characterize the resident microbiota in a field population of A. aegypti to evaluate its role associated with susceptibility to the insecticides permethrin and deltamethrin. Mosquitoes were fed 10% sucrose mixed with antibiotics and then exposed to insecticides using a diagnostic dose. DNA was extracted, and sequencing of bacterial 16S rRNA was carried out on Illumina® MiSeq™. Proteobacteria (92.4%) and Bacteroidetes (7.6%) were the phyla, which are most abundant in mosquitoes fed with sucrose 10%. After exposure to permethrin, the most abundant bacterial species were Fantoea agglomerans (38.4%) and Fseudomonas azotoformans-fluorescens-synxantha (14.2%). Elizabethkingia meningoseptica (38.4%) and Fs. azotoformans-fluorescens- synxantha (26.1%) were the most abundant after exposure to deltamethrin. Our results showed a decrease in mosquitoes' survival when exposed to permethrin, while no difference in survival when exposed to deltamethrin when the microbiota was modified. We found that the change in microbiota modifies the response of mosquitoes to permethrin. These results are essential for a better understanding of mosquito physiology in response to insecticides.
- Subjects
AEDES aegypti; PERMETHRIN; PYRETHROIDS; DELTAMETHRIN; CHIKUNGUNYA; YELLOW fever; VIRUS diseases; INSECTICIDES
- Publication
Frontiers in Microbiology, 2022, Vol 13, p1
- ISSN
1664-302X
- Publication type
Article
- DOI
10.3389/fmicb.2022.761459