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- Title
Effects of 15-deoxy-Δ<sup>12,14</sup>-prostaglandin J<sub>2</sub> on the expression of Toll-like receptor 4 and 2 in the murine lung in the presence of lipopolysaccharide.
- Authors
Inoue, Ken-ichiro; Takano, Hirohisa; Yanagisawa, Rie; Ichinose, Takamichi; Sadakane, Kaori; Yoshino, Shin; Yamaki, Kouya; Uchiyama, Kazuhiko; Yoshikawa, Toshikazu
- Abstract
1. Previously, we have demonstrated that 15-deoxy-Δ12,14-prostaglandin J2 (15d-PGJ2) enhances acute lung injury induced by lipopolysaccharide (LPS) in mice. The enhancement in acute lung injury by 15d-PGJ2 was concomitant with the enhanced expression of several pro-inflammatory cytokines in the lung. However, other underlying mechanisms of this enhancement remain to be elucidated. The present study investigated the effects of 15d-PGJ2 on the expression of Toll-like receptor (TLR) 4 and 2 in the lung in the absence or presence of LPS.2. In the present study, ICR mice were divided into four experimental groups that received (intratracheally) vehicle, LPS (125 μg/kg), 15d-PGJ2 (1 mg/kg) or 15d-PGJ2 + LPS. The mRNA expression of both TLR4 and 2 in the lung was evaluated 4 h after intratracheal administration.3. 15-Deoxy-Δ12,14-prostaglandin J2 enhanced the mRNA expression of both TLR4 and 2 in the presence of LPS.4. These results suggest that the enhancing effects of 15d-PGJ2 on LPS-induced acute lung injury may be explained, at least in part, by its effect on the lung expression of TLR4 and 2.
- Subjects
PROSTAGLANDINS; LUNGS; MICE; MESSENGER RNA; CYTOKINES; EVALUATION
- Publication
Clinical & Experimental Pharmacology & Physiology, 2005, Vol 32, Issue 3, p230
- ISSN
0305-1870
- Publication type
Article
- DOI
10.1111/j.1440-1681.2005.04175.x