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- Title
Physical and functional interaction between HCV core protein and the different p73 isoforms.
- Authors
Alisi, Anna; Giambartolomei, Stefania; Cupelli, Felicia; Merlo, Paola; Fontemaggi, Giulia; Spaziani, Alessandra; Balsano, Clara
- Abstract
Hepatitis C virus (HCV) core protein is a structural viral protein that packages the viral genomic RNA. In addition to this function, HCV core also modulates a number of cellular regulatory functions. In fact, HCV core protein has been found to modulate the expression of the cyclin-dependent inhibitor p21WAF1/CIP1 and to promote both apoptosis and cell proliferation through its physical interaction with p53. Here, we studied the ability of HCV core to bind the p53-related p73 protein, its isoforms and its deletion mutants. We found that HCV core co-immunoprecipitated with p73 in HepG2 and SAOS-2 cells. Deletion mutational analysis of p73 indicates that the domain involved in HCV core binding is located between amino-acid residues 321-353. We also demonstrate that p73/core interaction results in the nuclear translocation of HCV core protein either in the presence of the p73 a or p73 ? tumor-suppressor proteins. In addition, the interaction with HCV core protein prevents p73 a, but not p73 ? dependent cell growth arrest in a p53-dependent manner. Our findings demonstrate that HCV core protein may directly influence the various p73 functions, thus playing a role in HCV pathogenesis.Oncogene (2003) 22, 2573-2580. doi:10.1038/sj.onc.1206333
- Subjects
HEPATITIS C virus; VIRAL proteins; APOPTOSIS; CELL proliferation
- Publication
Oncogene, 2003, Vol 22, Issue 17, p2573
- ISSN
0950-9232
- Publication type
Article
- DOI
10.1038/sj.onc.1206333