We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Evaluation of Radioiodinated 1,4-Naphthoquinones as Necrosis Avid Agents for Rapid Myocardium Necrosis Imaging.
- Authors
Su, Chang; Zhang, Dongjian; Bao, Na; Ji, Aiyan; Feng, Yuanbo; Chen, Li; Ni, Yicheng; Zhang, Jian; Yin, Zhiqi
- Abstract
<bold>Purpose: </bold>Identifying necrotic myocardium in ischemic regions is of great importance for risk stratification and clinical decision-making. However, rapid noninvasive imaging of necrotic myocardium is still challenging. This study sought to evaluate the potential of 1,4-naphthoquinones to rapidly visualize necrotic myocardium and the possible mechanisms of necrosis avidity.<bold>Procedures: </bold>Six 1,4-naphthoquinones were radiolabeled with iodine-131 and the necrosis avidity was estimated in mouse models with muscular necrosis by gamma counting and autoradiography. The necrotic myocardium imaging property and biodistribution of [131I]naphthazarin (6) were determined in rat models with re-perfused myocardial infarction. A possible mechanism of necrosis avidity was explored by in vitro DNA-binding and in vivo blocking experiments.<bold>Results: </bold>The radiochemical purities of the six radiotracers were greater than 95 %. The uptakes in necrotic muscles of all six radiotracers were higher than those in viable muscles, and [131I]naphthazarin (6) showed the highest necrotic-to-viable ratio and necrosis-to-blood ratio at all tested time points. The necrotic myocardium could be clearly visualized by single-photon emission computed tomography/x-ray computed tomography (SPECT/CT) using [131I]naphthazarin (6) as early as 3 h post-injection. Post-mortem biodistribution showed the uptake of [131I]naphthazarin (6) in necrotic myocardium was 11.67-fold higher than that in viable myocardium. Absorption spectra and emission spectra suggested naphthazarin (6) could bind to DNA through intercalation. The uptake of [131I]naphthazarin (6) in necrotic muscle could be significantly blocked by excessive ethidium bromide (a typical DNA intercalator) and cold naphthazarin (6) with 63.49 and 71.96 % decline at 3 h post-injection in vivo, respectively.<bold>Conclusions: </bold>1,4-Naphthoquinones retained necrosis avidity and [131I]naphthazarin (6) rapidly visualized necrotic myocardium. The necrosis avidity mechanism of [131I]naphthazarin (6) may be attributed to its binding with exposed DNA in necrotic tissues.
- Subjects
RADIOIODINATION; NAPHTHOQUINONE; MYOCARDIAL infarction diagnosis; NECROSIS; MEDICAL decision making; DNA metabolism; ANIMAL experimentation; BIOLOGICAL models; COMPARATIVE studies; DIAGNOSTIC imaging; HIGH performance liquid chromatography; LIQUID chromatography; RESEARCH methodology; MEDICAL cooperation; MICE; MOLECULAR structure; MYOCARDIUM; QUINONE; RADIOGRAPHY; RATS; RESEARCH; SPECTROPHOTOMETRY; EVALUATION research; SINGLE-photon emission computed tomography; IODINE radioisotopes
- Publication
Molecular Imaging & Biology, 2018, Vol 20, Issue 1, p74
- ISSN
1536-1632
- Publication type
journal article
- DOI
10.1007/s11307-017-1089-3