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- Title
Molecular Study of Toxoplasma Gondii Infection in Sheep at Basrah Province.
- Authors
Al-Mothafer, Sahar Mohammed Ali; Awad, Abdul Hussein Habash
- Abstract
Toxoplasma gondii is the causative agent of toxoplasmosis, one of the most common parasitic infections of humans and pets. In this study, the parasite was isolated from chopped diaphragm muscles from naturally infected sheep in Basrah Province. 196 meat samples were collected from local sheep during the period from February 2020 to March 2021. The samples were examined microscopically and (115) positive samples containing the parasite were recorded. The percentage of infection was (59%). The current study included a genetic detection of T. gondii in the sheep to find out the infection rate spread of the parasite in local sheep meat and to determine the main cause of transmission of infection to humans. Nested Polymerase chain reaction technique using specific nested primers was used for genetic detection of the parasite in sheep. The PCR-positive samples recorded an infection rate of (28.8%). The DNA sequences were studied to determine the sequence of the nitrogenous bases of the DNA of the parasite. The genetic tree of these samples comparing with isolated hosts was drawn.
- Subjects
TOXOPLASMA gondii; SHEEP; BASE pairs; RESPIRATORY muscles; SHEEP parasites; SHEEP farming
- Publication
Journal of Pharmaceutical Negative Results, 2022, Vol 13, p716
- ISSN
0976-9234
- Publication type
Article
- DOI
10.47750/pnr.2022.13.S03.109