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- Title
Validation of the slaughterhouse porcine heart model for ex-situ heart perfusion studies.
- Authors
Kaffka genaamd Dengler, Selma E; Mishra, Mudit; van Tuijl, Sjoerd; de Jager, Saskia CA; Sluijter, Joost PG; Doevendans, Pieter A; van der Kaaij, Niels P
- Abstract
Introduction: To validate slaughterhouse hearts for ex-situ heart perfusion studies, we compared cold oxygenated machine perfusion in less expensive porcine slaughterhouse hearts (N = 7) to porcine hearts that are harvested following the golden standard in laboratory animals (N = 6). Methods: All hearts received modified St Thomas 2 crystalloid cardioplegia prior to 4 hours of cold oxygenated machine perfusion. Hearts were perfused with homemade modified Steen heart solution with a perfusion pressure of 20–25 mmHg to achieve a coronary flow between 100–200 mL/min. Reperfusion and testing was performed for 4 hours on a normothermic, oxygenated diluted whole blood loaded heart model. Survival was defined by a cardiac output above 3 L with a mean aortic pressure above 60 mmHg. Results: Both groups showed 100% functional survival, with laboratory hearts displaying superior cardiac function. Both groups showed similar decline in function over time. Conclusion: We conclude that the slaughterhouse heart can be used as an alternative to laboratory hearts and provides a cost-effective method for future ex-situ heart perfusion studies.
- Subjects
SWINE; BIOLOGICAL models; PEARSON correlation (Statistics); EXTRACORPOREAL membrane oxygenation; LABORATORY animals; T-test (Statistics); RESEARCH funding; XENOGRAFTS; HEART; ORGAN donation; MANN Whitney U Test; DESCRIPTIVE statistics; HEART transplantation; FOOD industry; KAPLAN-Meier estimator; LOG-rank test; ANALYSIS of variance; PERFUSION; DATA analysis software; CARDIAC surgery
- Publication
Perfusion, 2024, Vol 39, Issue 3, p555
- ISSN
0267-6591
- Publication type
Article
- DOI
10.1177/02676591231152718