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- Title
Residual muscle cytochrome c oxidase activity accounts for submaximal exercise lactate threshold in chronic progressive external ophthalmoplegia.
- Authors
Siciliano, Gabriele; Rossi, Bruno; Manca, Laura; Angelini, Corrado; Tessa, Alessandra; Vergani, Lodovica; Martinuzzi, Andrea; Muratorio, Alberto; Siciliano, G; Rossi, B; Manca, L; Angelini, C; Tessa, A; Vergani, L; Martinuzzi, A; Muratorio, A
- Abstract
The data from histological, biochemical, and mitochondrial DNA (mtDNA ) studies of muscle biopsies from 10 patients affected with chronic progressive external ophthalmoplegia (CPEO) were related to dynamic and metabolic parameters of incremental submaximal exercise. Maximum power output was reduced in all patients as compared to controls. Analysis of the venous lactate curve during exercise revealed a lactate threshold at exercise levels ranging from 40 to 50% of the predicted maximal power output. An earlier significant increase in lactate could be detected by calculating the mean delta lactate. Lactate values were inversely correlated with the cytochrome c oxidase (COX) activity of isolated muscle mitochondria. No relationship was found between lactate values and the number of ragged red fibers, or cytochrome c oxidase-negative fibers of the proportion of deleted mtDNA measured in muscle biopsy specimens. The discussion underscores the value of lactate kinetics in assessing skeletal muscle function, as well as the use of muscle COX levels to predict the effectiveness of wild-type complementation of deleted skeletal muscle mtDNA in in vivo contractile performance of CPEO subjects.
- Subjects
ENZYME metabolism; CARBOXYLIC acids; COMPARATIVE studies; DNA; DYNAMICS; EXERCISE; LACTATES; RESEARCH methodology; MEDICAL cooperation; GENETIC mutation; RESEARCH; RESEARCH funding; EVALUATION research; SKELETAL muscle; CHRONIC progressive external opthalmoplegia
- Publication
Muscle & Nerve, 1996, Vol 19, Issue 3, p342
- ISSN
0148-639X
- Publication type
journal article
- DOI
10.1002/(SICI)1097-4598(199603)19:3<342::AID-MUS10>3.0.CO;2-U