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- Title
Rat Preputial Sebocyte Differentiation Involves Peroxisome Proliferator-Activated Receptors<sup>1</sup>.
- Authors
Rosenfield, Robert L.; Kentsis, Alex; Deplewski, Dianne; Ciletti, Nancy
- Abstract
The hallmark of sebaceous epithelial cell (sebocyte) differentiation is the accumulation of fused neutral fat droplets. Very little sebocyte differentiation occurs, however, in primary or organ culture, even upon incubating with androgens, which are required for maturation in vivo. We hypothesized that sebocyte cell culture systems lack activators of the peroxisome proliferator-activated receptors that are involved in adipocyte differentiation. We here report that activation of peroxisome proliferator-activated receptor γ and α by their respective specific ligands, a thiazolidinedione and a fibrate, induced lipid droplet formation in sebocytes but not epidermal cells. Linoleic acid and carbaprostacyclin, both peroxisome proliferator-activated receptor δ and α ligand-activators, were more effective but less specific, stimulating lipid formation in both types of cells. Either was more effective than the combination of peroxisome proliferator-activated receptor γ and α activation, suggesting that peroxisome proliferator-activated receptor δ is involved in this lipid formation. Linoleic acid 0.1 mM stimulated significantly more advanced sebocyte maturation than any other treatment, including carbaprostacyclin, which suggests a distinct role of long chain fatty acids in sebocyte differentiation. Peroxisome proliferator-activated receptor γ1 mRNA was demonstrated in sebocytes, but not in epidermal cells; it was more strongly expressed in freshly dispersed than in cultured sebocytes. In contrast, peroxisome proliferator-activated receptor δ mRNA was expressed to a similarly high extent before and after culture in both sebocytes and epidermal cells. These findings are compatible with the concepts that peroxisome proliferator-activated receptor γ1 gene expression plays a unique role in the differentiation of sebocytes, while peroxisome proliferator-activated receptor δ activation and long chain fatty acids...
- Subjects
EPITHELIAL cells; PEROXISOMES; SKIN disease treatment; ACNE
- Publication
Journal of Investigative Dermatology, 1999, Vol 112, Issue 2, p226
- ISSN
0022-202X
- Publication type
Article
- DOI
10.1046/j.1523-1747.1999.00487.x