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- Title
Autoradiographic Evaluation of Monoclonal Antibodies Access to Melanoma-Associated Antigens in Melanoma Xenografts.
- Authors
Matzku, Siegfried; Schmid, Jörg; Tilgen, Wolfgang
- Abstract
Autoradiography of nude mice bearing human malignant melanoma xenografts was performed to characterize the distribution pattern of radioiodinated anti-melanoma monoclonal antibodies (MoAb) and fragments in macroscopic tumor modules. Non-uniformity of radioactivity distribution was seen in all MoAb-xenograft combinations. The predominant patterns were marked deposition of radioactivity either in the periphery of nodules or in sharply delimited intra-tumoral foci. These patterns were generated by limitations in the accessibility of melanoma tissue rather than gross necrosis or heterogeneity of antigen expression. Computer-aided densitometry of autoradiograms was used to elaborate the difference of accumulation in intra-tumoral hot spots versus cold areas. It was found that increasing uniformity was achieved by increasing the dose of MoAb (i.e., intact IgG) injected, whereas a reduction in the size of MoAb (IgG> F(ab')2> Fab) showed no such effect.
- Subjects
MONOCLONAL antibodies; MELANOMA; IMMUNOGLOBULINS; XENOGRAFTS; TRANSPLANTATION of organs, tissues, etc.; ANTIGENS; AUTORADIOGRAPHY
- Publication
Journal of Investigative Dermatology, 1990, Vol 95, Issue 6, p671
- ISSN
0022-202X
- Publication type
Article
- DOI
10.1111/1523-1747.ep12514342