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- Title
Urokinase- and Tissue-Type Plasminogen Activators in Keratinocytes During Wound Reepithelialization In Vivo.
- Authors
Grøndahl-Hansen, Jan; Lund, Leif R.; Ralfkiær, Elisabeth; Ottevanger, Vibeke; Danø, Keld
- Abstract
Urokinase- and tissue-type plasminogen activators (u-PA and t-PA) were identified immunohistochemically during re-epithelialization of mouse and human skin wounds, by means of polyclonal and monoclonal antibodies. In incised mouse skin wounds u-PA immunoreactivity was found in keratinocytes at the edge of the wound after 12 h, and at days 2 to 10 after wounding it was found in virtually all keratinocytes of the epithelial outgrowth that gradually covered the wound. At day 14, the epidermis appeared normal and no u-PA immunoreactivity was detected. t-PA immunoreactivity was found from day 5 to day 10 in some keratinocytes located superficially in the epidermal outgrowths near the edge of the mouse wounds. In 3- and 5-day old human skin wounds, u-PA immunoreactivity was found in keratinocytes in the epithelial outgrowths, whereas no t-PA immunoreactivity was detected. No u-PA and no t-PA immunoreactivity was found in normal mouse and human epidermis. The specificity of the staining was supported by a variety of controls, including absorption of the polyclonal antibodies with highly purified u-PA and t-PA preparations and zymographic analysis of extracts of wound tissue. The function of the plasminogen activators during reepithelialization is discussed and it is suggested that the keratinocytes use plasmin activated by u-PA for dissecting their way through the provisional matrix in the upper part of the granulation tissue.
- Subjects
UROKINASE; PLASMINOGEN activators; SKIN injuries; MONOCLONAL antibodies; KERATINOCYTES; EPIDERMIS
- Publication
Journal of Investigative Dermatology, 1988, Vol 90, Issue 6, p790
- ISSN
0022-202X
- Publication type
Article
- DOI
10.1111/1523-1747.ep12461511