We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Molecular Pro-Apoptotic Activities of Flavanone Derivatives in Cyclodextrin Complexes: New Implications for Anticancer Therapy.
- Authors
Adamus-Grabicka, Angelika A.; Hikisz, Pawel; Stepniak, Artur; Malecka, Magdalena; Paneth, Piotr; Sikora, Joanna; Budzisz, Elzbieta
- Abstract
This study evaluates the antiproliferative potential of flavanones, chromanones and their spiro-1-pyrazoline derivatives as well as their inclusion complexes. The main goal was to determine the biological basis of molecular pro-apoptotic activities and the participation of reactive oxygen species (ROS) in shaping the cytotoxic properties of the tested conjugates. For this purpose, changes in mitochondrial potential and the necrotic/apoptotic cell fraction were analyzed. Testing with specific fluorescent probes found that ROS generation had a significant contribution to the biological anticancer activity of complexes of flavanone analogues. TT (thrombin time), PT (prothrombin time) and APTT (activated partial tromboplastin time) were used to evaluate the influence of the compounds on the extrinsic and intrinsic coagulation pathway. Hemolysis assays and microscopy studies were conducted to determine the effect of the compounds on RBCs.
- Subjects
CYCLODEXTRIN derivatives; REACTIVE oxygen species; THROMBIN time; INCLUSION compounds; FLUORESCENT probes
- Publication
International Journal of Molecular Sciences, 2024, Vol 25, Issue 15, p8488
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms25158488