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- Title
Molecular basis of purine-rich RNA recognition by the human SR-like protein Tra2-β1.
- Authors
Cléry, Antoine; Jayne, Sandrine; Benderska, Natalya; Dominguez, Cyril; Stamm, Stefan; Allain, Frédéric H.-T.
- Abstract
Tra2-β1 is a unique splicing factor as its single RNA recognition motif (RRM) is located between two RS (arginine-serine) domains. To understand how this protein recognizes its RNA target, we solved the structure of Tra2-β1 RRM in complex with RNA. The central 5′-AGAA-3′ motif is specifically recognized by residues from the β-sheet of the RRM and by residues from both extremities flanking the RRM. The structure suggests that RNA binding by Tra2-β1 induces positioning of the two RS domains relative to one another. By testing the effect of Tra2-β1 and RNA mutations on the splicing of SMN2 exon 7, we validated the importance of the RNA-protein contacts observed in the structure for the function of Tra2-β1 and determined the functional sequence of Tra2-β1 in SMN2 exon 7. Finally, we propose a model for the assembly of multiple RNA binding proteins on this exon.
- Subjects
PURINES; RNA; ARGININE; CARRIER proteins; EXONS (Genetics)
- Publication
Nature Structural & Molecular Biology, 2011, Vol 18, Issue 4, p443
- ISSN
1545-9993
- Publication type
Article
- DOI
10.1038/nsmb.2001