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- Title
Correlation of somatic cell steroid secretion and quality of generated oocytes after in-vitro stimulation of mouse follicles.
- Authors
Hung-Ching Lju; Zhiying He; Zev Rosenwaks
- Abstract
Purpose : To test the possibility of follicular somatic cell steroidogenesis as a marker (or quality of their embraced oocytes. Methods: Mechanically isolated mouse preantral follicles were cultured and matured in-vitro (IVC/IVM) for study. Results: During IVC/IVM, oogenesis occurred concomitantly with folliculogenesis in a coordinated manner and simultaneously with progressive increments of somatic cell steroidogenesis. Follicular E2 production of matured oocytes were significantly higher than that of immature ones. The majority of MII oocytes (32/36) and all developed blastocysts(12/12) were associated with active E2 production prior to ovulation. In this study, 18 MII occytes met both requirements (or active and optimal E2 production. 13 of them were fertilized and 10 developed into blastocysts. Conclusion: Active somatic cell steroidogenesis prior to ovulation and an optimal steroid milieu at ovulation are prerequisites (or generation of competent oocytes after follicular maturation in-vitro.
- Subjects
SOMATIC cells; OVULATION; HUMAN reproduction; GENETICS; BIOLOGY
- Publication
Journal of Assisted Reproduction & Genetics, 2006, Vol 23, Issue 4, p191
- ISSN
1058-0468
- Publication type
Article
- DOI
10.1007/s10815-006-9041-5