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- Title
Laser phototherapy accelerates oral keratinocyte migration through the modulation of the mammalian target of rapamycin signaling pathway.
- Authors
Amorim Pellicioli, Ana Carolina; Domingues Martins, Manoela; Siviero Dillenburg, Caroline; Martins Marques, Márcia; Squarize, Cristiane H.; Castilho, Rogerio M.
- Abstract
Keratinocytes play a central role in wound healing by responding to tissue injury through the activation of cellular proliferation and migration. Current clinical evidence suggests that the laser phototherapy (LPT) accelerates wound healing in a variety of oral diseases; however, the molecular mechanisms involved in response to LPT are not fully understood. Oral keratinocytes (NOK-SI) maintained under nutritional-deficit culture medium (2%fetal bovine serum) were irradiated with InGaAlP laser (660 nm; 40 mW; 0.04 cm² spot size) in punctual and contact modes. The energy densities used were 4 and 20 J/cm² corresponding to 4 and 20 s of exposure times and 0.16 and 0.8 J of energy per point, respectively. Three sessions of irradiations were applied with 6-h intervals. Further, the impact of LPT over cellular migration, proliferation, and activation of the mammalian target of rapamycin (mTOR) pathway, known to play amajor role in epithelialmigration and wound healing, was analyzed. Compared with control cells, the LPT-treated cells showed accelerated cellular migration without any changes in proliferation. Furthermore, LPT resulted in an increase in the phospho-S6 ribosomal protein, indicating activation of the mTOR signaling pathway. Collectively, these findings suggest that the LPT activates mTOR signaling pathway, promotes epithelial cell migration, and accelerates healing of oral mucosa.
- Subjects
KERATINOCYTES; CELL proliferation; CANCER chemotherapy; RADIOTHERAPY; EPITHELIAL cells
- Publication
Journal of Biomedical Optics, 2014, Vol 19, Issue 2, p1
- ISSN
1083-3668
- Publication type
Article
- DOI
10.1117/1.JBO.19.2.028002