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- Title
Peritoneal fluid of women with endometriosis reduces <italic>SOD1</italic> in bovine oocytes in vitro maturation.
- Authors
Malvezzi, Helena; Da Broi, Michele Gomes; Meola, Juliana; Rosa-e-Silva, Júlio César; Ferriani, Rui Alberto; Navarro, Paula Andrea
- Abstract
Studies have demonstrated oxidative stress in peritoneal fluid (PF) from women with endometriosis and the importance of enzymatic antioxidant machinery to avoid oocyte oxidative damage. Considering that PF constantly surrounds the ovaries and has direct contact with the oocyte at ovulation, we wonder if PF from women with endometriosis may affect antioxidant enzyme gene expression. Thus, the present study aims to evaluate the PF impact from infertile women with minimal and mild endometriosis and from fertile control women without endometriosis on <italic>SOD1</italic>, <italic>CAT</italic>, <italic>GSR</italic> gene’s expression in experimental bovine oocytes matured in vitro. Samples of PF were obtained from women who underwent videolaparoscopy—7 infertile with EI/II and 7 fertile without endometriosis. Immature bovine oocytes underwent in vitro maturation in the absence of PF and in the presence of three concentrations (1, 5 and 10%) of PF from fertile and from infertile women with EI/II. After 22 to 24 h of IVM, oocytes were denuded and stored for analysis of <italic>SOD1</italic>, <italic>CAT</italic> and <italic>GSR</italic> by real-time polymerase chain reaction. Oocyte <italic>SOD1</italic> expression was significantly lower in the 10% endometriosis group (0.67 ± 0.32) when compared with no-peritoneal fluid (1.05 ± 0.24, <italic>p</italic> < 0.008) and 10% control groups (1.06 ± 0.22, <italic>p</italic> < 0.006). These findings raise the possibility of a deleterious influence of PF from women with EI/II on the oocyte, not only after ovulation but also during the maturation process, which could contribute to worsening oocyte quality, being one of the mechanisms related to infertility in patients with endometriosis.
- Subjects
ASCITIC fluids; ENDOMETRIOSIS; OVUM; OVULATION; DEVELOPMENTAL biology; OXIDATIVE stress; GENE expression; SUPEROXIDE dismutase; CATTLE
- Publication
Cell & Tissue Research, 2018, Vol 372, Issue 3, p621
- ISSN
0302-766X
- Publication type
Article
- DOI
10.1007/s00441-018-2805-2