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- Title
Fermentative production of 1-propanol from sugars using wild-type and recombinant Shimwellia blattae.
- Authors
Urano, Nobuyuki; Fujii, Misaki; Kaino, Hiroshi; Matsubara, Mitsuru; Kataoka, Michihiko
- Abstract
Shimwellia blattae is an enteric bacterium and produces endogenous enzymes that convert 1,2-propanediol (1,2-PD) to 1-propanol, which is expected to be used as a fuel substitute and a precursor of polypropylene. Therefore, if S. blattae could be induced to generate its own 1,2-PD from sugars, it might be possible to produce 1-propanol from sugars with this microorganism. Here, two 1,2-PD production pathways were constructed in S. blattae, resulting in two methods for 1-propanol production with the bacterium. One method employed the L-rhamnose utilization pathway, in which L-rhamnose is split into dihydroxyacetone phosphate and 1,2-PD. When wild-type S. blattae was cultured with L-rhamnose, an accumulation of 1,2-PD was observed. The other method for producing 1,2-PD was to introduce an engineered 1,2-PD production pathway from glucose into S. blattae. In both cases, the produced 1,2-PD was then converted to 1-propanol by 1,2-PD converting enzymes, whose production was induced by the addition of glycerol.
- Subjects
ENTEROBACTERIACEAE; BACTERIAL enzymes; PROPYLENE glycols; PROPANOLS; MICROBIOLOGICAL synthesis
- Publication
Applied Microbiology & Biotechnology, 2015, Vol 99, Issue 4, p2001
- ISSN
0175-7598
- Publication type
Article
- DOI
10.1007/s00253-014-6330-2