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- Title
Retinal-Based Anion Pump from the Cyanobacterium Tolypothrixcampylonemoides.
- Authors
Rokitskaya, Tatyana I.; Alekseev, Aleksey A.; Tsybrov, Fedor M.; Bukhalovich, Sergej M.; Antonenko, Yuri N.; Gordeliy, Valentin I.
- Abstract
In this work, TcaR rhodopsin from the cyanobacterium Tolypothrix campylonemoides was characterized. Analysis of the amino acid sequence of TcaR revealed that this protein possesses a TSD motif that differs by only one amino acid from the TSA motif of the known halorhodopsin chloride pump. The TcaR protein was expressed in E. coli, purified, and incorporated into proteoliposomes and nanodiscs. Functional activity was measured by electric current generation through the planar bilayer lipid membranes (BLMs) with proteoliposomes adsorbed on one side of the membrane surface, as well as by fluorescence using the voltage-dependent dye oxonol VI. We have shown that TcaR rhodopsin functions as a powerful anion pump. Our results show that the novel microbial anion transporter, TcaR, deserves deeper investigation and may be of interest both for fundamental studies of membrane proteins and as a tool for optogenetics.
- Subjects
AMINO acid sequence; BILAYER lipid membranes; ESCHERICHIA coli; ANIONS; MEMBRANE proteins
- Publication
Biochemistry (00062979), 2023, Vol 88, Issue 10, p1571
- ISSN
0006-2979
- Publication type
Article
- DOI
10.1134/S0006297923100127