We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Methanolic extract of Eryngium creticum Lam leaves, flowers and roots: Quantification and qualification of phenolic contents, antitumor effect and antioxidant capacity.
- Authors
NUSAIR, Shreen; AHMAD, Mohammad; EL-ELIMAT, Tamam; AL-ESSA, Khansaa; ABU-QATOUSEH, Luay; KHASAWNEH, Rawand
- Abstract
Natural products are potential sources of prospective antitumor agents. The phenolic and flavonoid contents of leaves, flowers, and roots of Eryngium creticum were quantified using liquid chromatography-tandem mass spectrometry (LC-ESI-MS/MS). The antioxidant capacity and antitumor activity of the three extracts were tested on MCF-7 and Hep2. The average total phenolic contents of E. creticum leaves, flowers, and roots were 12.43 ± 0.24, 10.09 ± 0.20, and 4.78 ± 0.10 mg of GAE/g of dw, respectively. The average total flavonoids were 4.77 ± 0.10, 6.23 ± 0.12, and 5.62 ± 0.12 mg of QE/g of dw, respectively. The extracts showed considerable DPPH? scavenger activity with average IC50 values of 1.41 ± 0.06, 2.70 ± 0.06, and 3.03 ± 0.05 µg/mL, respectively. The average IC50 values of scavenging capacity of NO? radicals were 0.11 ± 0.03, 0.69 ± 0.04, and 0.44 ± 0.01 µg/mL, respectively. The average IC50 values in MCF-7 were 1.25 ± 0.50, 3.75 ± 0.48, and 1.88 ± 0.28 mg/mL, respectively. In Hep2, the average IC50 values were 1.16 ± 0.58, 3.75 ± 0.79, and 1.31 ± 0.47 µg/mL, respectively. Phenolic content varied between the different extracts; leaves showed the highest levels of gallic acid (3.60 µg/mL), querciti-3-O-galactoside (0.82 µg/mL), cyanin chloride (1.52 µg/mL), and rosmarinic acid (4.65 µg/mL). Roots showed the lowest quantities of the measured phenolics, except for chlorogenic acid (6.00 µg/mL) and rosmarinic acid (1.86 µg/mL). The three extracts of E. creticum possess in vitro antitumor activities, which could be utilized as an adjunct with chemotherapy.
- Subjects
CHLOROGENIC acid; OXIDANT status; LIQUID chromatography-mass spectrometry; GALLIC acid
- Publication
Journal of Research in Pharmacy, 2022, Vol 26, Issue 4, p770
- ISSN
2630-6344
- Publication type
Article
- DOI
10.29228/jrp.174