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- Title
Assessing microvessels and angiogenesis in human breast cancer, using VE-cadherin.
- Authors
Martin, T. A.; Watkins, G.; Lane, J.; Jiang, W. G.
- Abstract
Martin T A, Watkins G, Lane J&Jiang W G(2005)HistopathologyAssessing microvessels and angiogenesis in human breast cancer, using VE-cadherin: Vascular endothelial (VE)-cadherin, also known as cadherin-5 and CD144, is an adhesion molecule uniquely expressed in endothelial cells. We hypothesized that VE-cadherin may be a useful marker for assessing microvessels and angiogenesis in human breast cancer and sought to determine whether a correlation exists between levels of VE-cadherin, angiogenic markers factor VIII and platelet endothelial cell adhesion molecule (PECAM)-1 and patient outcome in breast cancer.: Frozen sections from breast cancer primary tumours (tumourn = 114, backgroundn = 30) were immunostained with VE-cadherin, factor VIII and PECAM-1 antibodies and microvessel number was assessed. RNA was reverse transcribed and analysed by quantitative polymerase chain reaction (Q-PCR). VE-cadherin immunostaining showed a significant difference in microvessel number in tumour compared with background. There was no significant difference in the number of microvessels stained with PECAM-1 or factor VIII; there was increased staining of other structures within the sample and higher general background staining. Q-PCR revealed elevated levels of VE-cadherin and PECAM-1 in tumour samples compared with background tissue and in patients with a poor prognosis, as determined by the Nottingham Prognostic Index. There was no difference in levels with factor VIII. Both VE-cadherin and PECAM-1 had significantly reduced expression in lobular compared with ductal carcinomas: there was no difference with factor VIII.: Higher levels of angiogenic marker molecules in breast cancer may have an association with poor prognosis in patients. Moreover, VE-cadherin appears to be a preferable marker for such analysis.
- Subjects
BREAST cancer; CANCER patients; NEOVASCULARIZATION; VASCULAR endothelium; RNA
- Publication
Histopathology, 2005, Vol 46, Issue 4, p422
- ISSN
0309-0167
- Publication type
Article
- DOI
10.1111/j.1365-2559.2005.02104.x