We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Adaptation of an in‐house PCR for the detection of Helicobacter pylori and the mutations associated with macrolide resistance into ready‐to‐use PCR microwell strips.
- Authors
Bénéjat, Lucie; Ducournau, Astrid; Domingues‐Martins, Chloé; Lecoeur, Mélanie; Blosse, Alice; Mégraud, Francis; Bessède, Emilie; Lehours, Philippe
- Abstract
Background and Objectives: The present study describes the successful adaptation of an in‐house Polymerase Chain Reaction (PCR) for Helicobacter pylori detection coupled with the main mutations associated with resistance to clarithromycin in ready‐to‐use PCR microwell strips. Materials and Methods: These microwell strips can be used on LightCycler® 480, and are delivered with nine microliters of the reaction mixture dispensed into 8‐well microwell strips. An extraction control PCR targeting the β‐globin household gene is amplified in the same run as H pylori detection. Results and Conclusion: These microwell strips can be stored at −20°C for 1 year and left at room temperature and in the light for up to 4 h with no impact on the PCR results. Microwell strips can also undergo a thaw and refreeze cycle without impacting the PCR results. These PCR microwell strips are available for purchase from Eurogentec.
- Subjects
HELICOBACTER pylori; HELICOBACTER pylori infections; POLYMERASE chain reaction; CLARITHROMYCIN
- Publication
Helicobacter, 2021, Vol 26, Issue 6, p1
- ISSN
1083-4389
- Publication type
Article
- DOI
10.1111/hel.12855