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- Title
SIRT2 Plays Significant Roles in Lipopolysaccharides-Induced Neuroinflammation and Brain Injury in Mice.
- Authors
Wang, Ban; Zhang, Youjun; Cao, Wei; Wei, Xunbing; Chen, James; Ying, Weihai
- Abstract
Several recent studies have suggested seemingly contrasting roles of SIRT2 in inflammation: Our previous cell culture study has indicated that SIRT2 siRNA-produced decrease in SIRT2 levels can lead to significant inhibition of lipopolysaccharides (LPS)-induced activation of BV2 microglia, suggesting that SIRT2 is required for LPS-induced microglial activation. In contrast, some studies have suggested that SIRT2 deficiency can lead to increased inflammation. In our current study, we used a mouse model of neuroinflammation to determine the roles of SIRT2 in LPS-induced inflammation. We found that administration of SIRT2 inhibitor AGK2 can significantly decrease LPS-induced increases in CD11b signals and the mRNA of TNF-α and IL-6. We further found that AGK2 can block LPS-induced nuclear translocation of NFκB. In addition, our study has shown that AGK2 can decrease not only LPS-induced increase in TUNEL signals-a marker of apoptosis-like damage, but also LPS-induced increases in the levels of active Caspase-3 and Bax. Collectively, our current in vivo study, together with our previous cell culture study, has suggested that SIRT2 is required for LPS-induced neuroinflammation and brain injury.
- Subjects
ENCEPHALITIS; BRAIN injuries; LABORATORY mice; LIPOPOLYSACCHARIDES; MICROGLIA; CHROMOSOMAL translocation
- Publication
Neurochemical Research, 2016, Vol 41, Issue 9, p2490
- ISSN
0364-3190
- Publication type
Article
- DOI
10.1007/s11064-016-1981-2