We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Hepatic Uptake of Autologous Immune Complexes in the Rat.
- Authors
van der Laan-Klamer, S. M.; Harms, G.; Atmosoerodjo-Briggs, J.; Hoedemaeker, Ph. J.; Hardonk, M. J.
- Abstract
The hepatic uptake of autologous immune complexes (1Cx) was studied in Wistar rats immunized with bovine serum albumin (BSA). After attaining a suitable serum titre of rat anti- BSA Ig (RatABSA) they were injected intravenously with 2 mgBSA. The antibody levels were measured with an ELISA and the rate of disappearance of BSA from the circulation was studied by labelling the antigen with FITC. At various times after injection of BSA the uptake of 1Cx in the different types of liver cells was studied with the immunoperoxidase technique at the light microscope and ultrastructural levels. Within 5 min of injection with BSA 1Cx appeared to be bound and internalized by Kupffer cells, endothelial cells, and hepatocytes. After 2 h hepatocytes showed many large vacuoles containing 1Cx, whereas most microvilli had disappeared from the sinusoidal plasma membrane of these cells. In rats with a high antibody titre BSA had disappeared almost completely (92%) from the blood within 2 h. Rats with low antibody titre showed a lower disappearance rate of BSA. whereas a considerable amounl of antibody was still present in the blood after 2 h. We conclude that autologous 1Cx are bound and endocytosed by Kupffer cells, endothelial cells and hepatocytes of the liver. The affinity for the antigen and amount of the antibodies present in the serum influence the elimination rate of the antigen from the circulation. Uptake of autologous 1Cx by hepatocyles occurs only in the presence of large amounts of circulating 1Cx.
- Subjects
IMMUNOGLOBULINS; ALBUMINS; ABDOMEN; MACROPHAGES; LINEAR algebra; SERUM albumin
- Publication
Scandinavian Journal of Immunology, 1986, Vol 23, Issue 4, p441
- ISSN
0300-9475
- Publication type
Article