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- Title
Protective effect of pretreatment with propofol against tumor necrosis factor-α-induced hepatic insulin resistance.
- Authors
LONG ZHOU; LILIN WANG; BAOCHENG YANG; JINFENG ZENG; QINGGUO ZHANG; HONGYI LEI; SHIYUAN XU
- Abstract
Insulin resistance is common in critically ill patients and seriously affects their prognosis. The anesthetic propofol (2,6-diisopropylphenol) has been shown to cause insulin resistance in rats; however, the specific mechanism underlying this phenomenon remains unknown. Thus, the aim of the present study was to determine the molecular mechanism through which propofol influences insulin resistance in the liver. The current study assessed the effects of propofol on the phosphorylation level of key enzymes involved in the insulin signaling pathway, as well as the glycogen content in primary mouse hepatocytes. Propofol administration was demonstrated to considerably reduce the phosphorylation levels of Akt (Ser473) and glycogen synthase kinase (GSK)-3β (Ser9) in the primary mouse hepatocytes. In addition, propofol was shown to downregulate the phosphoinositide 3-kinase (PI3K)/Akt/GSK-3β signaling pathway and inhibit glycogen synthesis in hepatocytes. Thus, the present results indicated that propofol induced insulin resistance in primary mouse hepatocytes. Notably, pretreatment with propofol in tumor necrosis factor (TNF)-α-induced primary mouse hepatocytes with insulin resistance was demonstrated to alleviate the inhibitory effects of TNF-α on the PI3K/Akt/GSK-3β signaling pathway and glycogen synthesis. These results indicated that propofol exerts a protective effect against insulin resistance in primary mouse hepatocytes induced by TNF-α, indicating that propofol therapy may be clinically feasible to alleviate insulin resistance in critically ill patients.
- Subjects
PROPOFOL; INTRAVENOUS anesthetics; PHENOLS; TUMOR necrosis factors; INSULIN resistance
- Publication
Experimental & Therapeutic Medicine, 2015, Vol 10, Issue 1, p289
- ISSN
1792-0981
- Publication type
Article
- DOI
10.3892/etm.2015.2496