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- Title
Eliminating Glutamatergic Input onto Horizontal Cells Changes the Dynamic Range and Receptive Field Organization of Mouse Retinal Ganglion Cells.
- Authors
Ströh, Sebastian; Puller, Christian; Swirski, Sebastian; Hölzel, Maj-Britt; van der Linde, Lea I. S.; Segelken, Jasmin; Schultz, Konrad; Block, Christoph; Monyer, Hannah; Willecke, Klaus; Weiler, Reto; Greschner, Martin; Janssen-Bienhold, Ulrike; Dedek, Karin
- Abstract
In the mammalian retina, horizontal cells receive glutamatergic inputs from many rod and cone photoreceptors and return feedback signals to them, thereby changing photoreceptor glutamate release in a light-dependent manner. Horizontal cells also provide feedforward signals to bipolar cells. It is unclear, however,howhorizontal cell signals also affect the temporal, spatial,andcontrast tuning in retinal output neurons, the ganglion cells.Tostudy this,wegenerated a genetically modifiedmouseline inwhichweeliminated the lightdependencyof feedbackbydeleting glutamate receptors from mouse horizontal cells. This genetic modification allowed us to investigate the impact of horizontal cells on ganglion cell signaling independent of the actualmodeof feedback in the outer retina and without pharmacological manipulation of signal transmission. In control and genetically modified mice (both sexes), we recorded the light responses of transient OFF-+ retinal ganglion cells in the intact retina. Excitatory postsynaptic currents (EPSCs) were reduced and the cells were tuned to lower temporal frequencies and higher contrasts, presumably because photoreceptor output was attenuated. Moreover, receptive fields of recorded cells showed a significantly altered surround structure. Our data thus suggest that horizontal cells are responsible for adjusting the dynamic range of retinal ganglion cells and, together with amacrine cells, contribute to the center/surround organization of ganglion cell receptive fields in the mouse.
- Subjects
RETINAL ganglion cells; GLUTAMATE receptors; RECEPTIVE fields (Neurology); EXCITATORY postsynaptic potential; LABORATORY rats
- Publication
Journal of Neuroscience, 2018, Vol 38, Issue 8, p2015
- ISSN
0270-6474
- Publication type
Article
- DOI
10.1523/JNEUROSCI.0141-17.2018