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- Title
Tetramethylpyrazine Protects against Hydrogen Peroxide-Provoked Endothelial Dysfunction in Isolated Rat Aortic Rings: Implications for Antioxidant Therapy of Vascular Diseases.
- Authors
Xiaojia Ni; Siu Ling Wong; Chi Ming Wong; Chi Wai Lau; Xiaogeng Shi; Yefeng Cai; Yu Huang
- Abstract
Background and Objectives. Oxidative stress can initiate endothelial dysfunction and atherosclerosis. This study evaluated whether tetramethylpyrazine (TMP), the predominant active ingredient in Rhizoma LigusticiWallichii (chuanxiong), prevents endothelial dysfunction in a rat model of oxidative stress. Methods. Isolated rat aortic rings were pretreated with various drugs before the induction of endothelial dysfunction by hydrogen peroxide (H2O2). Changes in isometric tension were then measured in acetylcholine- (ACh-) relaxed rings. Endothelial nitric oxide synthase (eNOS) expression was evaluated in the rings by Western blotting, and superoxide anion (O2•-) content was assessed in primary rat aortic endothelial cells by dihydroethidium- (DHE-) mediated fluorescence microscopy. Results. ACh-induced endothelium-dependent relaxation (EDR) was disrupted by H2O2 in endothelium-intact aortic rings. H2O2-impaired relaxationwas ameliorated by acute pretreatment with lowconcentrations of TMP, as well as by pretreatment with catalase and the NADPH oxidase inhibitors, apocynin and diphenyleneiodonium (DPI). TMP, apocynin, and DPI also reduced O2•- accumulation in endothelial cells,but TMP failed to alter eNOS expression in aortic rings incubated with H2O2. Conclusions. TMP safeguards against oxidative stress-induced endothelial dysfunction, suggesting that the agent might find therapeutic utility in the management of vascular diseases. However, TMP's role in inhibiting NADPH oxidase and its vascular-protective mechanism of action requires further investigation.
- Subjects
CHINA; PHYTOTHERAPY; ANALYSIS of variance; ANIMAL experimentation; AORTA; ARTERIOSCLEROSIS; CELL culture; MUSCLE contraction; RATS; RESEARCH funding; WESTERN immunoblotting; OXIDATIVE stress; TREATMENT effectiveness; IN vitro studies
- Publication
Evidence-based Complementary & Alternative Medicine (eCAM), 2014, Vol 2014, p1
- ISSN
1741-427X
- Publication type
Article
- DOI
10.1155/2014/627181