We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
CTX-M-15-producing Escherichia coli clone B2-O25b-ST131 and Klebsiella spp. isolates in municipal wastewater treatment plant effluents.
- Authors
Dolejska, Monika; Frolkova, Petra; Florek, Magdalena; Jamborova, Ivana; Purgertova, Michaela; Kutilova, Iva; Cizek, Alois; Guenther, Sebastian; Literak, Ivan
- Abstract
Objectives The global occurrence of antibiotic resistance genes in bacteria in water environments is an increasing concern. Treated wastewater was sampled daily over a 45 day period from the outflow of a municipal wastewater treatment plant in Brno, Czech Republic, and examined for extended-spectrum β-lactamase (ESBL)-producing bacteria. Methods Water samples were cultivated on MacConkey agar with cefotaxime (2 mg/L) and individual colonies were examined for ESBL production. Phenotypic ESBL-positive bacteria identified as Escherichia coli or Klebsiella spp. were tested for the presence of antibiotic resistance genes, the virulence gene afa/dra and the blaCTX-M upstream region. Genetic relatedness was analysed by PFGE, multilocus sequence typing and plasmid analysis. Results A total of 68 ESBL-producing Enterobacteriaceae isolates were detected in 34 out of 45 wastewater samples. ESBL-producing isolates included 26 E. coli isolates, 4 Klebsiella pneumoniae isolates and 1 Klebsiella oxytoca isolate. The pandemic and multiresistant B2-O25b-ST131 clone was predominant, being detected among 19 E. coli isolates, and 17 of the B2-O25b-ST131 isolates were positive for the FIA replicon and the afa/dra operon and had an IS26 element flanking blaCTX-M-15. Seventeen of the B2-O25b-ST131 isolates showed closely related PFGE profiles (defined by 84% band similarity) and belonged to identical clonal groups. Conclusions The results highlight the inadequacy of the treatment process in removing multiresistant bacteria from municipal wastewater and point to a risk of transmission of clinically important multiresistant strains, such as the pandemic ST131 clone, to the environment. This is the first study demonstrating the pandemic ST131 clone in wastewater.
- Subjects
BRNO (Czech Republic); CZECH Republic; ANTIBIOTICS; DRUG resistance; WASTEWATER treatment; BETA lactamases; PLASMIDS; THERAPEUTICS
- Publication
Journal of Antimicrobial Chemotherapy (JAC), 2011, Vol 66, Issue 12, p2784
- ISSN
0305-7453
- Publication type
Article
- DOI
10.1093/jac/dkr363