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- Title
白藜芦醇对胰岛 β 细胞凋亡的影响及机制探讨.
- Authors
徐碧林; 俞秀华; 申甜; 喻明; 汪红平; 朱近悦
- Abstract
Objective To observe the effect of resveratrol on the apoptosis of mice insulinoma cell line β-TC-6 (hereinafter referred to as β cells) induced by hydrogen peroxide ( H2O2 ), and to explore its possible mechanism. Methods The pancreatic β cells were randomly divided into the observation group, control group and blank group, which were respectively cultured in medium with 10 μmol/L resveratrol + 200 μmol/L H2O2, 200 μmol/L H2O2 and non-H2O2 for 48 hours. The apoptosis rate of p cells and reactive oxygen species ( ROS ) were calculated by flow cytometry, superoxide dismutase ( SOD1 ) mRNA was measured by RT-PCR, and the expression of silent information regulator factor 2-related enzyme 1 ( SIRT1 ) and peroxisome proliferator-activated reccptor-γ coactivator 1α ( PGC- 1α) protein was determined by Western blotting. Results Comparison of the apoptosis rate: control group > observation group > blank group ( all P < 0. 05 ), comparison of SOD1 mRNA expression: blank group > observation group > control group ( all P < 0. 05 ), comparison of ROS levels, control group > observation group > blank group ( all P < 0. 05 ), and the comparison of SIRT1 protein : blank group > observation group > control group ( all P < 0. 01 ), the expression of PGC-la protein in the observation group and blank group was higher than that of the control group ( all P <0.01). Conclusions Resveratrol significantly reduces the apoptosis of p cells induced by H2O2. The mechanism may be related to the activation of SIRT1/PGC-1α pathways which leads to the increased SOD1 expression and decreased ROS expression.
- Publication
Shandong Medical Journal, 2015, Vol 55, Issue 46, p1
- ISSN
1002-266X
- Publication type
Article
- DOI
10.3969/j.issn.1002-266X.2015.46.001