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- Title
The smfBox is an open-source platform for single-molecule FRET.
- Authors
Ambrose, Benjamin; Baxter, James M.; Cully, John; Willmott, Matthew; Steele, Elliot M.; Bateman, Benji C.; Martin-Fernandez, Marisa L.; Cadby, Ashley; Shewring, Jonathan; Aaldering, Marleen; Craggs, Timothy D.
- Abstract
Single-molecule Förster Resonance Energy Transfer (smFRET) is a powerful technique capable of resolving both relative and absolute distances within and between structurally dynamic biomolecules. High instrument costs, and a lack of open-source hardware and acquisition software have limited smFRET's broad application by non-specialists. Here, we present the smfBox, a cost-effective confocal smFRET platform, providing detailed build instructions, open-source acquisition software, and full validation, thereby democratising smFRET for the wider scientific community. Broad uptake of smFRET has been hindered by high instrument costs and a lack of open-source hardware and acquisition software. Here, the authors present the smfBox, a cost-effective open-source platform capable of measuring precise FRET efficiencies between dyes on freely diffusing single molecules.
- Subjects
FLUORESCENCE resonance energy transfer; SINGLE molecules; SCIENTIFIC community
- Publication
Nature Communications, 2020, Vol 11, Issue 1, pN.PAG
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-020-19468-4