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- Title
SARS-CoV-2 inactivation in laboratory animal tissues with 4% formaldehyde or 5% glutaraldehyde for transfer to biosafety level 1 laboratories.
- Authors
Pilchová, Veronika; Elmontaser Mergani, Ahmed; Clever, Sabrina; Ciurkiewicz, Malgorzata; Becker, Kathrin; Gerhauser, Ingo; Baumgärtner, Wolfgang; Volz, Asisa; von Köckritz-Blickwede, Maren; Schulz, Claudia
- Abstract
The SARS-CoV-2 pandemic required the immediate need to transfer inactivated tissue from biosafety level (BSL)-3 to BSL-1 areas to enable downstream analytical methods. No validated SARS-CoV-2 inactivation protocols were available for either formaldehyde (FA)-fixed or glutaraldehyde (GA)-fixed tissues. Therefore, representative tissue from ferrets and hamsters was spiked with 2.2 × 106 tissue culture infectious dose 50% per ml (TCID50/ml) SARS-CoV-2 or were obtained from mice experimentally infected with SARS-CoV-2. SARS-CoV-2 inactivation was demonstrated with 4% FA or 5% GA at room temperature for 72 hours by a titer reduction of up to 103.8 TCID50/ml in different animal tissues with a maximum protein content of 100 µg/mg and a thickness of up to 10 mm for FA and 8 mm for GA. Our protocols can be easily adapted for validating the inactivation of other pathogens to allow for the transfer of biological samples from BSL-3 areas to BSL-1 laboratories.
- Subjects
LABORATORY animals; SARS-CoV-2; FORMALDEHYDE; GLUTARALDEHYDE; TISSUE culture; COVID-19 pandemic
- Publication
Veterinary Pathology, 2024, Vol 61, Issue 2, p201
- ISSN
0300-9858
- Publication type
Article
- DOI
10.1177/03009858231197128