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- Title
Precise mapping of the Goodpasture epitope(s) using phage display, site-directed mutagenesis, and surface plasmon resonance.
- Authors
Gozalbo-Rovira, Roberto; Rodríguez-Díaz, Jesús; Saus, Juan; Cervera, Javier
- Abstract
Goodpasture disease is an autoimmune disorder mediated by circulating autoantibodies against the noncollagenous-1 (NC1) domain of the α3 chain of type IV collagen (α3(IV)NC1). The structure of Goodpasture epitope(s) has been previously mapped into two main binding regions (EA and EB) of the α3(IV)NC1 domain using a residue mutation approach on the highly related α1(IV)NC1 domain. Here we combined phage display and surface plasmon resonance technology to more precisely localize the pathogenic binding sites. Peptides mimicking the Goodpasture epitope(s) were used to identify residues involved in autoantibody binding and found involvement of eight residues previously unrecognized within and outside of the EA or EB regions. Residue involvement in pathogenic reactivity was confirmed by site-directed mutagenesis on a more divergent α2(IV)NC1 molecule. From a mutant (M1) of the α2(IV)NC1 molecule, harboring residues previously identified as belonging to the Goodpasture epitope, additional chimeras were generated on the bases of phage display findings. All these mutants were shown to display higher reactivity with circulating Goodpasture autoantibodies than the M1 mutant. Thus, our results more precisely define Goodpasture epitope determinants and open new avenues to delineate comprehensive autoantibody-blocking agents for therapeutics.
- Subjects
EPITOPES; AUTOIMMUNE diseases; MUTAGENESIS; SURFACE plasmon resonance; AUTOANTIBODIES; COLLAGEN; THERAPEUTICS
- Publication
Kidney International, 2013, Vol 83, Issue 3, p438
- ISSN
0085-2538
- Publication type
Article
- DOI
10.1038/ki.2012.399